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mmlv reverse transcriptase 5× reaction buffer  (Promega)

 
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    Structured Review

    Promega mmlv reverse transcriptase 5× reaction buffer
    Mmlv Reverse Transcriptase 5× Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmlv+reverse+transcriptase+5%C3%97+reaction+buffer/mgcl2/pmc08228226-116-24-30
    Average 90 stars, based on 1 article reviews
    mmlv reverse transcriptase 5× reaction buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes
    Article Snippet: Extracted RNA was subjected to conventional reverse transcription (RT) for first-strand cDNA synthesis by mixing 0.5 μL of 500 ng/μL random hexamers (Promega, Madison, WI, USA), 1 μL of RNA (500 ng), and 2.25 μL of nuclease-free water to achieve a total volume of 3.75 μL. .. The mixture was then heated to 70 °C for 5 min before incubating on ice for 1 min. A total of 1.25 μL of MMLV reverse transcriptase 5× reaction buffer (Promega), 1.25 μL of dNTPs (10 μM), 0.16 μL of recombinant RNasin ribonuclease inhibitor (Promega), 0.25 μL of MMLV reverse transcriptase (Promega), and nuclease-free water were added to the mixture to give a final total reaction volume of 10 μL, and incubated at 37 °C for 1 h. Following first-strand synthesis, the cDNAs were diluted five times with nuclease-free water. .. Real-time PCR was then carried out for each sample using 5 μL of FastStart Essential DNA Green Master (Roche, Basel, Switzerland), 3 μL of nuclease-free water, 0.5 μL of target gene forward primer (10 μM), 0.5 μL of target gene reverse primer (10 μM) , and 1 μL of diluted cDNA.

    Recombinant:

    Article Title: Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes
    Article Snippet: Extracted RNA was subjected to conventional reverse transcription (RT) for first-strand cDNA synthesis by mixing 0.5 μL of 500 ng/μL random hexamers (Promega, Madison, WI, USA), 1 μL of RNA (500 ng), and 2.25 μL of nuclease-free water to achieve a total volume of 3.75 μL. .. The mixture was then heated to 70 °C for 5 min before incubating on ice for 1 min. A total of 1.25 μL of MMLV reverse transcriptase 5× reaction buffer (Promega), 1.25 μL of dNTPs (10 μM), 0.16 μL of recombinant RNasin ribonuclease inhibitor (Promega), 0.25 μL of MMLV reverse transcriptase (Promega), and nuclease-free water were added to the mixture to give a final total reaction volume of 10 μL, and incubated at 37 °C for 1 h. Following first-strand synthesis, the cDNAs were diluted five times with nuclease-free water. .. Real-time PCR was then carried out for each sample using 5 μL of FastStart Essential DNA Green Master (Roche, Basel, Switzerland), 3 μL of nuclease-free water, 0.5 μL of target gene forward primer (10 μM), 0.5 μL of target gene reverse primer (10 μM) , and 1 μL of diluted cDNA.

    Incubation:

    Article Title: Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes
    Article Snippet: Extracted RNA was subjected to conventional reverse transcription (RT) for first-strand cDNA synthesis by mixing 0.5 μL of 500 ng/μL random hexamers (Promega, Madison, WI, USA), 1 μL of RNA (500 ng), and 2.25 μL of nuclease-free water to achieve a total volume of 3.75 μL. .. The mixture was then heated to 70 °C for 5 min before incubating on ice for 1 min. A total of 1.25 μL of MMLV reverse transcriptase 5× reaction buffer (Promega), 1.25 μL of dNTPs (10 μM), 0.16 μL of recombinant RNasin ribonuclease inhibitor (Promega), 0.25 μL of MMLV reverse transcriptase (Promega), and nuclease-free water were added to the mixture to give a final total reaction volume of 10 μL, and incubated at 37 °C for 1 h. Following first-strand synthesis, the cDNAs were diluted five times with nuclease-free water. .. Real-time PCR was then carried out for each sample using 5 μL of FastStart Essential DNA Green Master (Roche, Basel, Switzerland), 3 μL of nuclease-free water, 0.5 μL of target gene forward primer (10 μM), 0.5 μL of target gene reverse primer (10 μM) , and 1 μL of diluted cDNA.



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